The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR goods had been digested and cloned into a pET21a+ vector using NdeI and XhoI restriction enzymes. A degree mutation was introduced by the short transform https://blu-78266542.dgbloggers.com/26935127/new-step-by-step-map-for-dimethandrolone-undecanoate